Journal: Bioactive Materials

Article Title: Exosome-functionalized photocrosslinked GelMA/HAMA hydrogel promotes facial nerve recovery via inflammatory microenvironment regulation

doi: 10.1016/j.bioactmat.2026.01.008

Figure Lengend Snippet: In vitro evaluation of immunomodulatory effects in composite hydrogel systems involving Nnat . (A) Top: Schematic representation of in vitro inflammatory model; Bottom: Morphological changes in macrophages observed via light microscopy. Scale bars: 100 μm. (B) Flow cytometric analysis of reactive oxygen species. (C) Phenotypic characterization of macrophage subtypes (M0/M1/M2) through surface marker detection. (D) Enhanced activation of NF-κB/P38 signaling pathways with elevated PI3K phosphorylation observed in Hydrogel + si- Nnat group versus hydrogel control. (E) Quantitative PCR analysis of gene expression. (F) Metabolic activity assessment through viability assays. (G) Apoptotic cell quantification. (H) TUNEL staining patterns across experimental groups. Scale bars: 50 μm. (I) Cytokine profile analysis using ELISA. (n = 5, ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.)

Article Snippet: Reaction systems (20 μL volume) were assembled in RNase-free tubes, incorporating cDNA templates, paired forward/reverse primers (10 μM concentration per primer), Vazyme's 2 × Taq Pro Universal SYBR qPCR Master Mix, and molecular-grade water.

Techniques: In Vitro, Light Microscopy, Marker, Activation Assay, Protein-Protein interactions, Phospho-proteomics, Control, Real-time Polymerase Chain Reaction, Gene Expression, Activity Assay, TUNEL Assay, Staining, Enzyme-linked Immunosorbent Assay

Journal: Non-coding RNA Research

Article Title: Exosomal miRNA-218–5p derived from low-passage dermal papilla cells modulates hair follicle growth and development

doi: 10.1016/j.ncrna.2026.01.004

Figure Lengend Snippet: Exosomes derived from low-passage DPCs regulated HFSC proliferation. (A) Indirect immunofluorescence showing ALPL and PCNA expression in low-passage (P1) and high-passage (P8) DPCs (scale bar = 50 μm). (B) RT-qPCR analysis of HF development-related gene expression in P1 and P8 DPCs (unpaired two-tailed t -test, n = 3). (C) TEM images of exosomes from P1 DPCs (DPC-Exos P1) and P8 DPCs (DPC-Exos P8). (D) NTA measurement of particle size of DPC-Exos P1 and DPC-Exos P8. (E) Western blot detection of exosome-specific proteins in DPC-Exos P1 and DPC-Exos P8. (F) CCK-8 assay evaluating HFSC proliferation after treatment with DPC-Exos from P1 and P8 (one-way ANOVA, n = 5). (G) Flow cytometry analysis of HFSC apoptosis after treatment with DPC-Exos from P1 and P8 (one-way ANOVA, n = 3). ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: According to the manufacturer's instructions, the ChamQ SYBR qPCR Master Mix (Vazyme, China, Cat No. Q311-02) on a QuantStudio® 5 instrument (Applied Biosystems) was used for reverse transcription-quantitative PCR (RT-qPCR).

Techniques: Derivative Assay, Immunofluorescence, Expressing, Quantitative RT-PCR, Gene Expression, Two Tailed Test, Western Blot, CCK-8 Assay, Flow Cytometry

Journal: Non-coding RNA Research

Article Title: Exosomal miRNA-218–5p derived from low-passage dermal papilla cells modulates hair follicle growth and development

doi: 10.1016/j.ncrna.2026.01.004

Figure Lengend Snippet: Differentially expressed miRNAs between DPC-Exos P1 and DPC-Exos P8 identified by miRNA sequencing. (A) Differential expression analysis of miRNAs between DPC-Exos P1 and DPC-Exos P8. (B) RT-qPCR validation of differential miRNA expression (unpaired two-tailed t -test, n = 3). (C) GO enrichment of target genes of DE miRNAs. (D) KEGG enrichment of target genes of DE miRNAs. ∗∗ P < 0.01.

Article Snippet: According to the manufacturer's instructions, the ChamQ SYBR qPCR Master Mix (Vazyme, China, Cat No. Q311-02) on a QuantStudio® 5 instrument (Applied Biosystems) was used for reverse transcription-quantitative PCR (RT-qPCR).

Techniques: Sequencing, Quantitative Proteomics, Quantitative RT-PCR, Biomarker Discovery, Expressing, Two Tailed Test

Journal: Non-coding RNA Research

Article Title: Exosomal miRNA-218–5p derived from low-passage dermal papilla cells modulates hair follicle growth and development

doi: 10.1016/j.ncrna.2026.01.004

Figure Lengend Snippet: RNA sequencing identified differentially expressed genes (DEGs) in HFSCs regulated by exosomal miR-218-5p. (A) Analysis of DEGs by RNA sequencing. (B) RT-qPCR validation of DEG expression levels (unpaired two-tailed t -test, n = 3). (C) GO enrichment of DEGs. (D) KEGG enrichment of DEGs. (E) Venn diagram showing overlap between downregulated DEGs and predicted miR-218–5p target genes. ∗∗ P < 0.01.

Article Snippet: According to the manufacturer's instructions, the ChamQ SYBR qPCR Master Mix (Vazyme, China, Cat No. Q311-02) on a QuantStudio® 5 instrument (Applied Biosystems) was used for reverse transcription-quantitative PCR (RT-qPCR).

Techniques: RNA Sequencing, Quantitative RT-PCR, Biomarker Discovery, Expressing, Two Tailed Test